For decades, mice have been the major transgenic in vivo model for gene function studies and drug discovery. Their low maintenance cost and easy manipulation made them a preferred tool for pre-clinical research. However, as the need for a better understanding of human genetics has grown, there has been an increased demand for improved transgenic in vivo models. Site-specific, transgenic rats are gaining importance as a preferred in vivo model for researchers who seek a better representation of human genetics and physiology. Their similarity to human physiology and pathology makes them a better in vivo model than mice for the study of cardiovascular, diabetes, neurological, psychiatric, and autoimmune dysfunctions.
Major improvements in site-directed mutagenesis using techniques such as TARGATTTM, CRISPR/Cas9, ZFNs and TALENs have made the generation of transgenic rat models possible. Applied StemCell’s proprietary TARGATTTM technology enables the insertion of any gene of interest (up to 20 kb) with high efficiency and guaranteed expression. The gene is integrated into a well-characterized, transcriptionally active locus in the rat genome engineered with an attP docking site The DNA is injected directly into the rat zygote, thereby bypassing rat embryonic stem cells (rES). Our innovative technology can generate site-specific, single copy knock-in rat in 4 months.
We recently presented the TARGATTTM-rat model and related services at the prestigious 13th Transgenic Technology Meeting held in Prague, Czech Republic from March 20-13, 2016. Download a copy of the poster
To learn more about the site-specific knock-in TARGATTTM-Rat Model, Register for our upcoming webinar.
Integrase-based TARGATTTM Method for Generating Site-Specific Transgenic Rat Models
Date: Tuesday, April 12, 2016
Time: 11:00 am – 12:00 pm
Presented by: Dr. Ruby Yanru Chen-Tsai (CSO, Senior VP, R&D), Applied StemCell, Inc.
The novel TARGATTTM technology and the complementary CRISPR/Cas9 system provide a versatile gene editing tool set for the generation of:
- Knock-in, Knock-out, and Conditional Knock-out Rat Models
- Knock-in, Knock-out, and Conditional Knock-out Mouse Models
- Gene editing in Cell lines and iPSCs